abstract：PURPOSE:To evaluate the inhibitory effect of human fetal retinal pigment epithelium (HFRPE) on the activation of human T-cells. METHODS:Pure cultures of HFRPE cells were incubated with purified human T-cells in three different activation assays: 1) allogenic peripheral blood mononuclear cells; 2) OKT3 coated beads in ...

journal_title：Current eye research

authors： Rezai KA,Semnani RT,Farrokh-Siar L,Hamann KJ,Patel SC,Ernest JT,van Seventer GA

更新日期：1999-06-01 00:00:00

abstract：PURPOSE:To determine by immunohistochemistry the distribution of different nitric oxide synthases (NOS) isoforms in the porcine eye. METHODS:By light microscopy (immunofluorescence), porcine ocular tissues were investigated using monoclonal antibodies against neuronal NOS (nNOS; NOS I), endothelial NOS (ecNOS; NOS III...

journal_title：Current eye research

authors： Meyer P,Champion C,Schlötzer-Schrehardt U,Flammer J,Haefliger IO

更新日期：1999-05-01 00:00:00

abstract：PURPOSE:Androgens are thought to play a role in the regulation of the human lacrimal gland. Androgen receptor mRNA has been isolated from human lacrimal tissue; however, it is not known which cell(s) in human lacrimal tissue may contain androgen receptors. This study is an immunohistochemical investigation of the locat...

journal_title：Current eye research

authors： Smith RE,Taylor CR,Rao NA,Young LL,Rife LL

更新日期：1999-04-01 00:00:00

abstract：PURPOSE:To evaluate the intraocular distribution and metabolism of the lipid prodrug of foscarnet, 1-O-octadecyl-sn-glycerol-3- phosphonoformate (ODG-PFA), following intravitreal administration. METHODS:Twenty rabbits received ODG-[14C]PFA intravitreal injection, yielding 0.632 mM resultant intravitreal concentration....

journal_title：Current eye research

authors： Cheng L,Hostetler KY,Gardner MF,Avila CP Jr,Bergeron-Lynn G,Severson GM,Freeman WR

更新日期：1999-03-01 00:00:00

abstract：PURPOSE:This study was undertaken in order to investigate whether the production of vasodilatory prostaglandins is increased in the retinal vasculature of the diabetic rat. METHODS:Diabetes was induced in male Sprague-Dawley rats using streptozotocin. Diabetic rats were left uncontrolled for 3-4 weeks or were treated ...

journal_title：Current eye research

authors： Johnson EI,Dunlop ME,Larkins RG

更新日期：1999-02-01 00:00:00

abstract：PURPOSE:After corneal injury, persistent epithelial defects (PED's) may occur due to the chronic failure of the regenerating epithelium to adhere to the underlying stroma. The aim of this study was to examine the potential of epsilonaminocaproic acid (EACA) as a topical treatment for PED's. EACA inhibits the activation...

journal_title：Current eye research

authors： Williams PB,Crouch ER Jr,Crouch ER,Mazaheri M

更新日期：1999-02-01 00:00:00

abstract：PURPOSE:To examine the effects of external modulation of epithelial-mesenchymal interaction on conjunctival epithelial cell differentiation characteristics. METHODS:Keratin K4 and laminin beta2 chain protein localization was examined in an organotypic model which facilitates the comparison of differentiation character...

journal_title：Current eye research

authors： Kurpakus MA,Lin L

更新日期：1999-01-01 00:00:00

abstract：PURPOSE:The experiments were designed to provide details on the characteristics of rhodopsin regeneration in the rod photoreceptors of diabetic mice and to evaluate their mechanistic basis. METHODS:A genetically-derived diabetic albino mouse was developed. An excised albino mouse eye preparation was used. The preparat...

journal_title：Current eye research

authors： Ostroy SE

更新日期：1998-10-01 00:00:00

abstract：PURPOSE:To examine the effect of elevated extracellular glucose, thus simulating diabetes, on migration, adhesion and proliferation of SV40 transformed human corneal epithelial (HCE) cells. METHODS:HCE cells were maintained in serum supplemented media containing 5 mM, 17.5 mM or 38 mM D-glucose. Cell migration was det...

journal_title：Current eye research

authors： McDermott AM,Kern TS,Murphy CJ

更新日期：1998-09-01 00:00:00

abstract：PURPOSE:Acanthamoeba keratitis is a sight-threatening corneal infection. It is known that: (i) more amoebae bind to the surface of injured corneas than to the normal corneal surface and (ii) mannose-containing glycoproteins (GPs) possess binding sites for Acanthamoeba. The present study was undertaken to determine whet...

journal_title：Current eye research

authors： Jaison PL,Cao Z,Panjwani N

更新日期：1998-08-01 00:00:00

abstract：PURPOSE:Intrastromal epithelial migration occurs following deep excimer annular keratectomy. Our purpose is to determine the localization of gelatinase B expression and its relationship to migrating intrastromal epithelial cells following deep annular excimer keratectomy. METHODS:Rabbit corneas were treated with deep ...

journal_title：Current eye research

authors： Maeda M,Vanlandingham BD,Ye H,Lu PC,Azar DT

更新日期：1998-08-01 00:00:00

abstract：PURPOSE:Previous studies have suggested that corneal fibrosis controlled by a TGFbeta-mediated cytocrine pathway underlies the development of clinical corneal haze and associated regression of photoablative effect following excimer laser PRK. Using a unique blocking antibody, we evaluated the role of TGFbeta in post-PR...

journal_title：Current eye research

authors： Møller-Pedersen T,Cavanagh HD,Petroll WM,Jester JV

更新日期：1998-07-01 00:00:00

abstract：PURPOSE:Hypercarbia has been suggested as a risk factor for retinopathy of prematurity (ROP). We investigated the effect of hypercarbia on the retinal vasculature of the neonatal rat to determine whether hypercarbia alone could induce preretinal neovascularization analogous to ROP. METHODS:In a preliminary blood gas s...

journal_title：Current eye research

authors： Holmes JM,Zhang S,Leske DA,Lanier WL

更新日期：1998-06-01 00:00:00

abstract：PURPOSE:Several procedures in cataract surgery carry the risk of high temperature increases in the capsular bag. The present study was undertaken to determine the shrinkage temperature of the human lens capsule and to investigate the effect of temperature on the mechanical behavior of the lens capsule. METHOD:Thermal-...

journal_title：Current eye research

authors： Krag S,Danielsen CC,Andreassen TT

更新日期：1998-05-01 00:00:00

abstract：PURPOSE:Endothelin-1 (ET-1) is a potent vasoconstrictive and neural peptide that has been demonstrated to be present and functionally active and important in the eye. This study was undertaken to examine for the first time the cellular distribution of ET-1 in the whole human eye. METHODS:Twelve human eyes were examine...

journal_title：Current eye research

authors： Wollensak G,Schaefer HE,Ihling C

更新日期：1998-05-01 00:00:00

abstract：PURPOSE:To determine the cellular mechanisms involved in the hypoxia-induced alteration of the retinal pigment epithelium (RPE) potentials and the light-evoked responses of the RPE in chicks. In addition, to determine the mechanisms involved in the recovery of the RPE during the post-hypoxic period. METHODS:In vitro p...

journal_title：Current eye research

authors： Nakazaki S,Nao-i N,Sawada A

更新日期：1998-04-01 00:00:00

abstract：PURPOSE:To determine whether environmental factors or bacterial viability affect the binding of two strains of P. aeruginosa to mouse cornea. METHODS:Scarified corneas were placed in organ culture and inoculated with P. aeruginosa cell suspensions containing either ATCC 19660 or PAO1 bacterial strains classed as cytot...

journal_title：Current eye research

authors： Chen L,Hobden JA,Masinick SA,Hazlett LD

更新日期：1998-03-01 00:00:00

abstract：PURPOSE:To examine the effects of nitric oxide synthase (NOS) inhibitors on intraocular pressure (IOP) and ocular inflammation following laser irradiation of the rabbit iris, and to investigate the involvement of nitric oxide (NO). METHODS:Thirty min after the intravenous administration of a nonselective inhibitor of ...

journal_title：Current eye research

authors： Taniguchi T,Kawakami H,Sawada A,Iwaki M,Tsuji A,Sugiyama K,Kitazawa Y

更新日期：1998-03-01 00:00:00

abstract：PURPOSE:We wished to establish which matrix metalloproteinases (MMPs) and metalloproteinase inhibitors (TIMPs) were present in human interphotoreceptor matrix (IPM) and vitreous. METHODS:IPM and vitreous were obtained from postmortem human eyebank eyes. Western immunoblots were probed with antibodies against human MMP...

journal_title：Current eye research

authors： Plantner JJ,Smine A,Quinn TA

更新日期：1998-02-01 00:00:00

abstract：PURPOSE:To investigate the characteristics of anterior chamber inflammatory reaction induced by intravitreal injection of endothelin-1 (ET-1). METHODS:The time course of changes in aqueous protein concentration (APC) after intravitreal injection of 10(-4), 10(-5), 10(-6) and 10(-7) M ET-1 into rabbit eyes was measured...

journal_title：Current eye research

authors： Shoji N,Oshika T,Masuda K

更新日期：1998-02-01 00:00:00

abstract：PURPOSE:The repair of the corneal epithelium is modulated by matrix metalloproteinases. The present study examined the effects of recombinant (r-) tissue inhibitors of metalloproteinases-1 and -2 (TIMP-1 and -2) on the proliferation of cultured epithelial cells from rabbit cornea, and on the spreading of a sheet of squ...

journal_title：Current eye research

authors： Saika S,Kawashima Y,Okada Y,Tanaka SI,Yamanaka O,Ohnishi Y,Ooshima A

更新日期：1998-01-01 00:00:00

abstract：PURPOSE:To investigate the biomechanical effect induced by radial keratotomy on outflow facility in paired, enucleated whole porcine eyes. METHODS:Freshly enucleated porcine eyes were perfused at a constant pressure of 10 mm Hg. Radial keratotomy (RK), with a 3.5 mm central clear zone and eight radial incisions, was p...

journal_title：Current eye research

authors： Becker HH,Eisenberg DL,Wang N,Steinert RF,Schuman JS

更新日期：1997-12-01 00:00:00

abstract：PURPOSE:To determine retinal vessel diameter variations in response to neuronal activity induced by diffuse luminance flicker. METHODS:The diameter of retinal arteries and veins was measured in 9 normal subjects by computer analysis of fundus pictures taken in monochromatic light under normal conditions of illuminatio...

journal_title：Current eye research

authors： Formaz F,Riva CE,Geiser M

更新日期：1997-12-01 00:00:00

abstract：PURPOSE:To investigate the effect of shaking on corneal endothelial preservation. METHODS:Thirty-six dog corneal buttons were obtained under standard eye bank procedure, after the animals had been sacrificed for cardiovascular experiments in the surgical department. They were equally divided into two groups. In Group ...

journal_title：Current eye research

authors： Wang IJ,Hu FR

更新日期：1997-11-01 00:00:00

abstract：PURPOSE:Form-deprivation myopia (FDM) is believed to result principally from actions of substances that modulate information processing in the retina. We used a chick model to investigate what role nitric oxide (NO), a gaseous neuromodulator, might play in the development of FDM. METHODS:We injected different concentr...

journal_title：Current eye research

authors： Fujikado T,Kawasaki Y,Fujii J,Taniguchi N,Okada M,Suzuki A,Ohmi G,Tano Y

更新日期：1997-10-01 00:00:00

abstract：PURPOSE:The effects of repeated topical administration of the selective beta 2-adrenergic agonist fenoterol on the intraocular pressure and on the adenylyl cyclase activity in ciliary processes in rabbit were examined in order to detect their possible causal relationship. METHODS:Intraocular pressure was measured by p...

journal_title：Current eye research

authors： Cepelík J,Hynie S

更新日期：1997-10-01 00:00:00

abstract：PURPOSE:Most studies of cell growth of the RPE employ cultures which have been previously passaged, but here we investigated freshly-explanted RPE cells, which may have been growth-quiescent for years, within the eye to determine whether co-culture affects initial outgrowth in primary culture. METHODS:Bovine or human ...

journal_title：Current eye research

authors： McKay BS,Burke JM

更新日期：1997-09-01 00:00:00

abstract：PURPOSE:To determine whether substance P is present in human tears. METHODS:Tear samples (1-2 microliters) were collected from one eye of each of 12 subjects. Two of the eyes had dry eye syndrome, two wore contact lenses and had dry eye syndrome, and eight were normal. Five of the eight normal eyes were scheduled to u...

journal_title：Current eye research

authors： Varnell RJ,Freeman JY,Maitchouk D,Beuerman RW,Gebhardt BM

更新日期：1997-09-01 00:00:00

abstract：PURPOSE:To characterize the effects that mode of sampling and overnight eye closure have on the nature of caseinolytic activity recovered in tear fluid. METHODS:Reflex, open and closed (R, O and C) eye tear fluids were collected by microcapillary tubes or from the inferior formix by Schirmer strip. Microcapillary coll...

journal_title：Current eye research

authors： Sakata M,Sack RA,Sathe S,Holden B,Beaton AR

更新日期：1997-08-01 00:00:00

abstract：PURPOSE:Corneal wound healing after excimer laser photorefractive keratectomy (PRK) passes through a series of characteristic stages which have earlier been defined by means of histological, histochemical, and biochemical approaches. We investigated the potential of confocal microscopy to verify morphological changes i...

journal_title：Current eye research

authors： Linna T,Tervo T

更新日期：1997-07-01 00:00:00

abstract：PURPOSE:Chloride efflux plays an important role in aqueous humor production. Chloride currents have been described in bovine non-pigmented ciliary epithelium (NPE), in transformed cultured human NPE, and in bovine volume-activated chloride channels described in the latter. It is the basolateral membranes of the NPE of ...

journal_title：Current eye research

authors： Chen S,Sears M

更新日期：1997-07-01 00:00:00

abstract：PURPOSE:Several previous studies have shown that glycoconjugates of extracellular matrix, cell membrane and nucleus play an important role in the mediation of cell proliferation, migration and differentiation. Lens epithelial cells and lens fiber cells show regional differences with regard to these parameters. If glyco...

journal_title：Current eye research

authors： Denk PO,Breipohl W,Naib-Majani W,Knorr M

更新日期：1997-06-01 00:00:00

abstract：PURPOSE:Histidine is an indispensable amino acid with an imidazole ring and it is a precursor of histamine, carnosine, and anserine. Histidine has been proposed to act as a neurotransmitter or neuromodulator in mammalian central nervous system (CNS), including retina. In this study, we report histidine-like immunoreact...

journal_title：Current eye research

authors： Kusakari Y,Nishikawa S,Ishiguro S,Tamai M

更新日期：1997-06-01 00:00:00

abstract：PURPOSE:The purpose of these studies is to develop an in vitro model of corneal endothelial aging and to investigate age-related changes in morphology, mitosis, prostaglandin synthesis and prostaglandin response pathways. METHODS:First-passage rabbit corneal endothelial cells were grown in vitro for up to 30 days afte...

journal_title：Current eye research

authors： Jumblatt MM

更新日期：1997-05-01 00:00:00

abstract：PURPOSE:A quick, simple, and efficient extraction technique was developed for the removal of protein from soft hydrophilic contact lenses. METHODS:An extraction solvent consisting of a 50:50 mix of 0.2% trifluoroacetic acid and acetonitrile was used to remove protein from in vitro laboratory-deposited and human-worn c...

journal_title：Current eye research

authors： Keith D,Hong B,Christensen M

更新日期：1997-05-01 00:00:00

abstract：PURPOSE:It has been demonstrated that chick eye growth compensates for defocus imposed by spectacle lenses: the eye elongates in response to hyperopic defocus imposed by negative lenses and slows its elongation in response to myopic defocus imposed by positive lenses. We ask whether the synthesis of scleral extracellul...

journal_title：Current eye research

authors： Nickla DL,Wildsoet C,Wallman J

更新日期：1997-04-01 00:00:00

abstract：PURPOSE:The fibroblast growth factors (FGFs) are a family of 9 heparin binding proteins which have been proposed to play key roles in angiogenesis. Basic FGF (bFGF), acidic FGF (aFGF) and FGF5 have previously been demonstrated to be expressed in the normal retina and RPE. In this study, the expression of FGF5 was explo...

journal_title：Current eye research

authors： Kitaoka T,Morse LS,Schneeberger S,Ishigooka H,Hjelmeland LM

更新日期：1997-04-01 00:00:00

abstract：PURPOSE:To compare the abilities of balanced salt solution, BSS Plus and Hartmann's lactated Ringer's (HLR) solution to maintain the viability of retinal vascular endothelial cells (RVEC) in vitro. METHODS:Cultured retinal vascular endothelial cells were suspended in each irrigating solution for four hours. Viability ...

journal_title：Current eye research

authors： Mourad KM,Edelhauser HF,Capone A Jr,Lynn MJ,Geroski DH

更新日期：1997-03-01 00:00:00

abstract：PURPOSE:Extracellular matrix homeostasis is dependent in part upon a family of matrix metalloproteinases and their specific inhibitors, the tissue inhibitors of metalloproteinases (TIMPs). Recently, gene defects in TIMP-3 have been identified in the affected individuals of several families with Sorsby's fundus dystroph...

journal_title：Current eye research

authors： Vranka JA,Johnson E,Zhu X,Shepardson A,Alexander JP,Bradley JM,Wirtz MK,Weleber RG,Klein ML,Acott TS

更新日期：1997-02-01 00:00:00

abstract：PURPOSE:Retinal ischemia and neovascularization (NV) are important components of many retinal disorders. To facilitate further investigation of retinal ischemia and neovascularization, we sought to develop a reproducible in vivo experimental model of venous occlusion by photodynamic thrombosis in rats. METHODS:After a...

journal_title：Current eye research

authors： Saito Y,Park L,Skolik SA,Alfaro DV,Chaudhry NA,Barnstable CJ,Liggett PE

更新日期：1997-01-01 00:00:00